A standard experimental diet for the study of fatty acid requirements of weaning and first ongrowing stages of European sea bass (Dicentrarchus labrax L.): selection of the basal diet
A standard experimental diet was developed to study lipid nutrition in marine fish larvae during weaning and first ongrowing. Basal diets were prepared by extrusioncooking, crumbling of the pellets, and sieving to obtain particles in the size ranges 150-300 |i.m and 300-500 fxm. Three protein sources in the extruded basal diet were evaluated: (1) micronized cod protein granulate (COD); (2) a mixture of casein, gelatin, and albumin (CGA); (3) a mixture of cod fish meal, whey protein, soybean protein concentrate, albumin, hemoglobin meal, and wheat gluten (MIX). A variable lipid fraction was added to the extruded nucleus by coating the particles with an emulsion, coconut oil (COCO), or fish oil emulsion (FO), in a fluidized bed granulator. European sea bass (Dicentrarchus labrax L.) were weaned and consequently reared for 3 weeks on the experimental diets in a recirculation system. A commercial diet and the uncoated COD diet served as control diets. The suitability of the diets was assessed on the basis of the water stability of the particles, survival, growth, resistance to higher salinity stress of the fish, and tissue fatty acid composition. The MIX diet may hold promise as a standard basal diet for lipid nutrition research with marine fish species during weaning and first ongrowing. Furthermore, the MIX basal diet coated with the appropriate lipid fraction may serve as a standard reference diet for comparison among fish species, laboratories, and between experiments.
Article from Marine Science Symposia Vol. 201 - "Mass rearing of juvenile fish". Symposium held in Bergen, 21-23 June 1993. To access the remaining articles please click on the keyword "MSS Vol. 201".