Determination of CYP1A-dependent mono-oxygenase activity in dab by fluorimetric measurement of EROD activity in S9 or microsomal liver fractions

Stagg, R.; McIntosh, A.; Gubbins, M. J.

doi:10.17895/ices.pub.5085

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Determination of CYP1A-dependent mono-oxygenase activity in dab by fluorimetric measurement of EROD activity in S9 or microsomal liver fractions

report

posted on 2016-01-01, 00:00 authored by R. Stagg, A. McIntosh, M. J. Gubbins

This paper describes a method for the determination of cytochrome P4501A (CYP1A) in fish liver fractions by the measurement of 7-ethoxyresorufin O-deethylase (EROD) activity. The proposed method is by fluorescent assay of resorufin using internal standard

History

Published under the auspices of the following ICES Steering Group or Committee

HAPISG

Published under the auspices of the following ICES Expert Group or Strategic Initiative

WGBEC

Series

ICES Techniques in Marine Environmental Science (TIMES)

Volume

57

ISBN

978-87-7482-168-7

ISSN

2707-6997

Recommended citation

[Authors]. 2016. Determination of CYP1A-dependent mono-oxygenase activity in dab by fluorimetric measurement of EROD activity in S9 or microsomal liver fractions. ICES Techniques in Marine Environmental Science, Vol. 57. [##] pp. https://doi.org/10.17895/ices.pub.5085