Genetic studies of families of Arcto-Norwegian and Norwegian coastal cod: preliminary random amplified polymorphic deoxyribonucleic acid studies
Information about reproductive success is difficult to obtain, since observation of spawning behaviour may be deceptive. Certain molecular biological methods could make such results easily obtainable. In this study RAPD (Random Amplified Polymorphic DNA) is examined as a way of determining parentage. PCR (Polymerase Chain Reaction) amplifications with short primers produce many fragments that can be exceedingly variable between individuals. Twenty-three of the 60 primers (10-mers) used produced readable profiles, and two produced diagnostic bands which may be used to exclude incorrect relationships.
Article from Marine Science Symposia Vol. 198 - "Cod and climate change". Symposium held in Reykjavik, 23-27 August 1993. To access the remaining articles please click on the keyword "MSS Vol. 198".