International Council for the Exploration of the Sea
ICES Marine Science Symposia - Volume 201 - 1995 - Part 25 of 67.pdf (2.24 MB)

Recent advances in biological aspects of mass culture of rotifers (Brachionus plicatilis) in Japan

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posted on 2023-07-11, 09:54 authored by Kazutsugu Hirayama, Atsushi Hagiwara

This paper summarizes fundamental research on improved rotifer culture technology in Japan. (1) Classification of Brachionus plicatilis strains into L- and S-types was supported by cross-mating experiments. We succeeded in crossing rotifers in each strain type belonging within L or S, but failed to obtain fertilized eggs in crosses made between L- and S-type strains. (2) Mass production of the resting eggs of L-type rotifers was conducted in 50 m'1 tanks by feeding the rotifers with baker's yeast and frozen Nannochloropsis oculata. Ten-day cultures produced about 2.3 x 109 resting eggs. The number of resting eggs produced by 1 g dry weight of food ranged from 87 to 149 x 103 eggs. (3) High-density rotifer mass culture technology is being developed by feeding condensed suspensions of freshwater Chlorella, which is commercially avail­able (15 x 109 cells/ml, vitamin B 12 1 mg/l), to rotifers cultured in 1 m3 tanks. Two nylon filter mats (1.1 x 2 m, 1 cm thickness) washed daily, were inserted into each tank to remove solids such as rotifer faeces. The rotifer culture could supply 5.9 x 108 rotifers d -1 throughout a 40-d culture period and maintain a rotifer density of more than 1000 individuals m l-1 .

Article from Marine Science Symposia Vol. 201 - "Mass rearing of juvenile fish". Symposium held in Bergen, 21-23 June 1993. To access the remaining articles please click on the keyword "MSS Vol. 201". 



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Hirayama, K., and Hagiwara, A. 1995. Recent advances in biological aspects of mass culture of rotifers (Brachionus plicatilis) in Japan. ICES Marine Science Symposia, 201: 153-158.

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